Journal: bioRxiv

Article Title: Direct effects on endothelial cells are essential for perivascular cell (pericyte)-dependent amplification of orthoflavivirus NS1-mediated microvascular leakage

doi: 10.64898/2026.01.29.702573

Figure Lengend Snippet: (A-B) ECIS measurement of permeability of monocultures of HUVECs (A) or LSECs (B) treated with preconditioned media from HSCs treated with 1 µg/mL NS1 or 100 ng/mL TNF-α for 24 h; “Untreated” refers to HUVECs or LSECs treated with HSC media only and the “EC only” refers to untreated HUVECs or LSECs without the addition of HSC media. (C, D) Dextran permeability assay measuring HUVEC (C) or LSEC (D) permeability in coculture with HSCs 24 h post-treatment with 1 µg/mL NS1, 100 ng/mL TNF-α (positive control), or eluate (negative) control. Data points represent technical replicates within an experiment with different symbol shapes corresponding to different experiments (N=3, n=3). Bars represent the mean, and error bars represent SEM. (E, F) TEER measurement of semi-contact cocultures of HUVECs (A) or LSECs (B) cultured with HSCs and treated with 1 µg/mL NS1, 100 ng/mL TNF-α (positive control), or eluate (negative) control. Permeability measurements were normalised to each sample pre-treatment for ECIS data (A, B) or the untreated endothelial cell only control (EC only; dashed line) for EVOM data (E, F). In the time course experiments (A, B, E, F), each data point represents the mean ± SEM. In all experiments indicated significance is compared to untreated cocultures; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Human umbilical vein endothelial cells (HUVECs) were obtained from PromoCell (Heidelberg, Germany); liver sinusoidal endothelial cells (LSECs) from Innoprot (Derio, Spain), and human hepatic stellate cells (HSCs) from Zen-Bio (Durham, NC USA).

Techniques: Permeability, FITC-Dextran Permeability Assay, Positive Control, Negative Control, Cell Culture, Control

Journal: bioRxiv

Article Title: Direct effects on endothelial cells are essential for perivascular cell (pericyte)-dependent amplification of orthoflavivirus NS1-mediated microvascular leakage

doi: 10.64898/2026.01.29.702573

Figure Lengend Snippet: Endothelial barrier integrity measurements for HUVEC (A, B) or LSEC (C) monocultures following treatment with 1 µg/mL wild-type DENV-2, AHFV or KFDV NS1, or the nonfunctional DENV-2 NS207Q mutant (negative control), or 100 ng/mL TNF-α (positive control), or eluate (negative) control as indicated. Permeability measurements were normalised to the untreated endothelial cell only control (Untreated) for EVOM TEER data (A), or to each sample before treatment for ECIS data (B, C). Each data point represents the mean ± SEM. Indicated significance is for treatment versus untreated EC only control (Untreated). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Human umbilical vein endothelial cells (HUVECs) were obtained from PromoCell (Heidelberg, Germany); liver sinusoidal endothelial cells (LSECs) from Innoprot (Derio, Spain), and human hepatic stellate cells (HSCs) from Zen-Bio (Durham, NC USA).

Techniques: Mutagenesis, Negative Control, Positive Control, Permeability, Control

Journal: bioRxiv

Article Title: Vascularized tumor organoids enable immunotherapy testing in tumor-remodeled stroma

doi: 10.64898/2026.01.23.701300

Figure Lengend Snippet: a, Endothelial activation by TNFα induces robust up-regulation of adhesion molecules across the microvascular network, shown as intensity heat maps for ICAM1 and VCAM1 (Control vs TNFα). b, Representative confocal images of the vascularized channel showing endothelial cells (cyan) and PBMCs (magenta/red). TNFα priming increases leukocyte tethering/adhesion and transendothelial migration (white dashed outlines; higher-magnification insets). c, Quantification of leukocyte–vessel interactions per chip, partitioned into adherent (grey) and extravasated (magenta) cells; TNFα markedly elevates both behaviors (bars = mean, error bars = s.d.; points = individual chips). d, Secreted CXCL10 measured from chip effluents rises after TNFα stimulation (points = chips; bars = mean ± s.d.). Data are representative of independent chips per condition.

Article Snippet: Human liver sinusoidal microvascular endothelial cells (LSMVECs) (Angio-Proteomie) expressing GFP were cultured in EGM-2 MV (Lonza) up to passage 9.

Techniques: Activation Assay, Control, Migration

Journal: bioRxiv

Article Title: Vascularized tumor organoids enable immunotherapy testing in tumor-remodeled stroma

doi: 10.64898/2026.01.23.701300

Figure Lengend Snippet: a, Time-lapse montage of a microvascular network co-cultured with healthy colon organoids during perfusion of fluorescent 70-kDa dextran (pseudocolor; yellow/white = higher intensity) at 0, 100, 200 and 300 s,showing rapid intraluminal filling adjacent to organoids. b, Wide-field views of the perfused channel along the chip with (top) and without (bottom) organoids illustrate uniform tracer distribution throughout the network. Images are representative of independent chips.

Article Snippet: Human liver sinusoidal microvascular endothelial cells (LSMVECs) (Angio-Proteomie) expressing GFP were cultured in EGM-2 MV (Lonza) up to passage 9.

Techniques: Cell Culture